Nowatorski automatyczny test immunologiczny dla przeciwciał

Ocena odpowiedzi immunogennej na nowatorską szczepionkę z koniugatem enterobaktyny u kurcząt do produkcji przeciwciał żółtka jaja gwinicznych dla enterobaktyny

Bierna immunizacja specyficznymi przeciwciałami żółtka jaja   (immunoglobulina Y, IgY) staje się obiecującą alternatywą dla antybiotyków do zwalczania infekcji bakteryjnych. Niedawno opracowaliśmy nową szczepionkę skoniugowaną, która może wywołać u królików silną odpowiedź immunologiczną skierowaną przeciwko enterobaktynie (Ent), wysoce konserwatywnej cząsteczce sideroforu wykorzystywanej przez różne patogeny Gram-ujemne. Jednak  wydaje się, że na indukcję przeciwciał swoistych dla Ent  wpływa wybór żywiciela zwierzęcego i reżim szczepień.

Nadal nie wiadomo, czy skoniugowana szczepionka Ent może wywołać specyficzną odpowiedź immunologiczną u niosek w celu wytworzenia przeciwciał IgY przeciwko żółtku jaja Ent. W tym badaniu przeprowadzono trzy próby szczepienia kurczaków z różnymi schematami w celu określenia warunków wydajnej produkcji przeciwciał IgY przeciwko żółtku jaja Ent . Oczyszczony Ent  skoniugowano odpowiednio  z trzema białkami nośnikowymi, hemocyjaniną skałoczepa (KLH), albuminą surowicy bydlęcej (BSA) i CmeC (kandydatem na podjednostkową szczepionkę).

Domięśniowa immunizacja warstw Barred Rock koniugatem KLH-Ent czterokrotnie wywołała silną odpowiedź immunologiczną przeciwko całej szczepionce skoniugowanej, ale miano przeciwciał IgY swoistych dla Ent nie zmieniło się w żółtku z jedynie 4-krotnym wzrostem w surowicy. W drugim badaniu oceniono trzy różne skoniugowane szczepionki Ent u kurek rasy Rhode Island Purple z czterema wstrzyknięciami podskórnymi. Koniugat KLH-Ent lub CmeC-Ent konsekwentnie indukował wysoki poziom przeciwciał IgY swoistych dla Ent zarówno w surowicy (do 2048 razy), jak iw żółtku (do 1024 razy) u każdego kurczaka . Jednak specyficzna dla Ent odpowiedź immunologiczna była wywoływana tylko tymczasowo i umiarkowanie za pomocą szczepienia BSA-Ent.

W trzecim badaniu dziesięć niosek rasy White Leghorn immunizowano podskórnie trzykrotnie KLH-Ent, co doprowadziło do spójnej i silnej odpowiedzi immunologicznej przeciwko zarówno całemu koniugatowi, jak i cząsteczce Ent u każdego kurczaka; średnie miano przeciwciał IgY swoistych dla Ent wzrosło odpowiednio o około 32 i 256 razy w surowicy i żółtku. Zgodnie z silnym wiązaniem z różnymi pochodnymi Ent, specyficzne dla Ent IgY żółtka jaja hamowały również   wzrost in vitro reprezentatywnego   szczepu Escherichia coli .

W sumie to badanie wykazało, że nowatorska szczepionka skoniugowana Ent może wywołać silną, specyficzną i silną odpowiedź immunologiczną u kurczaków. Specyficzne dla Entów hiperimmunizacyjne IgY żółtka jaja mają potencjał do biernej interwencji immunologicznej przeciwko infekcjom Gram-ujemnym.

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Human Tubulin beta- 4 chain, TUBB4 ELISA KIT

ELI-13731h 96 Tests
EUR 988.8

Donkey anti Goat IgG (H + L) (Alexa Fluor 594)

43R-ID005AF 500 ug
EUR 405.6
Description: Donkey anti Goat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID022AF 500 ug
EUR 436.8
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Rat IgG (H + L) (Alexa Fluor 594)

43R-ID047AF 500 ug
EUR 554.4
Description: Donkey anti Rat IgG (H + L) secondary antibody (Alexa Fluor 594)

Donkey anti Chicken IgY (H + L) (Alexa Fluor 594)

43R-ID056AF 500 ug
EUR 411.6
Description: Donkey anti Chicken IgY secondary antibody (H + L) (Alexa Fluor 594)

Rabbit anti Chicken IgY (H + L) (Alexa Fluor 594)

43R-IR016AF 1 mg
EUR 337.2
Description: Rabbit anti Chicken IgY (H + L) secondary antibody (Alexa Fluor 594)

XFD594 Anti-human CD32 Antibody *IV.3, XFD594 Same Structure to Alexa Fluor™ 594*

10320170 100 tests
EUR 245

XFD594 Anti-human CD32 Antibody *IV.3, XFD594 Same Structure to Alexa Fluor™ 594*

10320171 500 tests
EUR 918

ELISA kit for Human Tubulin beta-4 chain (TUBB4)

KTE60116-48T 48T
EUR 398.4
Description: Quantitative sandwich ELISA for measuring Human Tubulin beta-4 chain (TUBB4) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Tubulin beta-4 chain (TUBB4)

KTE60116-5platesof96wells 5 plates of 96 wells
EUR 2538
Description: Quantitative sandwich ELISA for measuring Human Tubulin beta-4 chain (TUBB4) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Tubulin beta-4 chain (TUBB4)

KTE60116-96T 96T
EUR 646.8
Description: Quantitative sandwich ELISA for measuring Human Tubulin beta-4 chain (TUBB4) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

iFluor® 594 Styramide *Superior Replacement for Alexa Fluor 594 tyramide*

45035 100 Slides
EUR 334

Anti-gamma-Tubulin Alexa Fluor647

A6-465-C025 0.025 mg
EUR 260.4

Anti-gamma-Tubulin Alexa Fluor647

A6-465-C100 0.1 mg
EUR 472.8

Donkey anti Goat IgG (H + L) (Fab 2) (Alexa Fluor 594)

43R-ID012AF 300 ug
EUR 492
Description: Donkey anti Goat IgG (H + L) secondary antibody (Fab'2) (Alexa Fluor 594)

Donkey Anti-Goat IgG (H+L), Alexa Fluor® 594 Conjugated

Ab8011-001 1mg
EUR 400.8

AF594-streptavidin conjugate [Streptavidin, Alexa Fluor™ 594 Conjugate]

16892 1 mg
EUR 211.2

AF594 Phalloidin [equivalent to Alexa Fluor® 594 phalloidin]

23158 300 Tests
EUR 367.2

Anti-Beta-tubulin Antibody

A1216-100
EUR 483.6

Anti-beta-Tubulin Purified

11-251-C025 0.025 mg
EUR 135.6

Anti-beta-Tubulin Purified

11-251-C100 0.1 mg
EUR 223.2

Anti-beta-Tubulin Purified

11-427-C025 0.025 mg
EUR 135.6

Anti-beta-Tubulin Purified

11-427-C100 0.1 mg
EUR 223.2

Anti-beta-Tubulin Purified

11-444-C025 0.025 mg
EUR 135.6

Anti-beta-Tubulin Purified

11-444-C100 0.1 mg
EUR 223.2

Anti-beta Tubulin antibody

PAab00879 100 ug
EUR 463.2

Anti-Beta Tubulin antibody

PAab00880 100 ug
EUR 463.2

anti- Beta Tubulin antibody

LSMab09872 100 ug
EUR 463.2

anti-beta-Tubulin (5G3)

LF-MA20322 100 ug
EUR 400.8
Description: Mouse monoclonal to betA tubulin

anti-beta-tubulin (M6)

LF-MA20425 100 ug
EUR 400.8
Description: Mouse monoclonal to Beta tubulin

anti-beta II tubulin

LF-MA20432 100 ug
EUR 400.8
Description: Mouse monoclonal to beta II tubulin

anti- beta Tubulin antibody

FNab00879 100µg
EUR 658.5
Description: Antibody raised against beta Tubulin

anti- Beta Tubulin antibody

FNab00880 100µg
EUR 658.5
Description: Antibody raised against Beta Tubulin

anti- Tubulin-beta antibody

FNab09105 100µg
EUR 658.5
Description: Antibody raised against Tubulin-beta

anti- Beta Tubulin antibody

FNab09872 100µg
EUR 658.5
Description: Antibody raised against Beta Tubulin

Anti-Tubulin beta antibody

STJ98436 100 µl
EUR 280.8
Description: Mouse monoclonal to Tubulin beta.

Anti-Tubulin beta antibody

STJ98566 100 µl
EUR 280.8
Description: Mouse monoclonal to Tubulin beta.

Anti-beta-Tubulin antibody

STJ99132 200 µl
EUR 236.4
Description: Mouse monoclonal to beta-Tubulin.

Anti-beta-Tubulin antibody

STJ99133 200 µl
EUR 236.4
Description: Mouse monoclonal to beta-Tubulin.

Anti-Tubulin beta antibody

STJ96145 200 µl
EUR 236.4
Description: Beta tubulin is a protein encoded by the tubb gene which is approximately 49,7 kDa. Beta tubulin is localised to the cytoskeleton and cytoplasm. It is involved in the regulation of PLK1 activity at G2/M transition, development of Slit-Robo signalling, the innate immune system, cell cycle and organelle biogenesis and maintenance. Beta tubulin contains a highly acidic C-terminal region which can bind cations such as calcium. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain and forms part of the cytoskeleton. Beta tubulin is ubiquitously expressed in the spleen, thymus and immature brain. Mutations in the tubb gene result in complex cortical dysplasia with other brain malformations. Mutations can also cause congenital symmetric circumferential, an autosomal dominant disease which results in multiple rings of folded skin mostly affecting the limbs. STJ96145 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Tubulin beta protein.

Anti-beta-Tubulin antibody

STJ96932 200 µl
EUR 236.4
Description: Beta tubulin is a protein encoded by the tubb gene which is approximately 49,7 kDa. Beta tubulin is localised to the cytoskeleton and cytoplasm. It is involved in the regulation of PLK1 activity at G2/M transition, development of Slit-Robo signalling, the innate immune system, cell cycle and organelle biogenesis and maintenance. Beta tubulin contains a highly acidic C-terminal region which can bind cations such as calcium. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain and forms part of the cytoskeleton. Beta tubulin is ubiquitously expressed in the spleen, thymus and immature brain. Mutations in the tubb gene result in complex cortical dysplasia with other brain malformations. Mutations can also cause congenital symmetric circumferential, an autosomal dominant disease which results in multiple rings of folded skin mostly affecting the limbs. STJ96932 was developed from clone 1E1-E8-H4 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody detects endogenous beta tubulin.

Anti-beta-Tubulin antibody

STJ97016 200 µl
EUR 236.4
Description: Mouse monoclonal to beta-Tubulin.

Anti-beta-tubulin antibody

STJ97037 200 µl
EUR 236.4
Description: Beta tubulin is a protein encoded by the tubb gene which is approximately 49,7 kDa. Beta tubulin is localised to the cytoskeleton and cytoplasm. It is involved in the regulation of PLK1 activity at G2/M transition, development of Slit-Robo signalling, the innate immune system, cell cycle and organelle biogenesis and maintenance. Beta tubulin contains a highly acidic C-terminal region which can bind cations such as calcium. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain and forms part of the cytoskeleton. Beta tubulin is ubiquitously expressed in the spleen, thymus and immature brain. Mutations in the tubb gene result in complex cortical dysplasia with other brain malformations. Mutations can also cause congenital symmetric circumferential, an autosomal dominant disease which results in multiple rings of folded skin mostly affecting the limbs. STJ97037 was developed from clone M7. The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous beta tubulin.

Anti-beta-tubulin antibody

STJ97041 200 µl
EUR 236.4
Description: Mouse monoclonal to beta-tubulin.

Anti-beta Tubulin antibody

STJ97131 200 µl
EUR 236.4
Description: Rabbit polyclonal to beta Tubulin.

Anti-Tubulin Beta antibody

STJ31562 200 µl
EUR 236.4
Description: Unonjugated rabbit polyclonal to tubulin beta

Anti-beta Tubulin antibody

STJ13100428 150 µl
EUR 512.4

Anti-Tubulin beta antibody

STJ13100435 500 µg
EUR 512.4

Anti-Beta-Tubulin antibody

STJ16100474 100 µg
EUR 487.2

anti-beta II Tubulin

YF-PA23016 100 ug
EUR 483.6
Description: Rabbit polyclonal to beta II Tubulin

anti-beta II Tubulin

YF-PA25563 50 ul
EUR 400.8
Description: Mouse polyclonal to beta II Tubulin

Human Kinase Library IV

HKIN-IV 1 set
EUR 540

alpha Tubulin (alpha Tubulin) Antibody (ATTO 594)

abx446844-100ug 100 ug
EUR 693.6

Beta-Tubulin

MO22180 100 ug
EUR 522

Recombinant (E.Coli) Human Thyrostimulin Beta

RP-594 10 ug
EUR 343.2

Anti-RPSA Alexa Fluor® 488

A4-829-C100 0.1 mg
EUR 372

Anti-CD40 antibody (Alexa-fluor 488)

STJ170000 100 µg
EUR 471.6
Description: CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40L (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40, predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin

Anti-LAMP3 antibody (Alexa-fluor 488)

STJ170004 100 µg
EUR 471.6
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-LAMP3 antibody (Alexa-fluor 546)

STJ170005 100 µg
EUR 471.6
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-LAMP3 antibody (Alexa-fluor 647)

STJ170006 100 µg
EUR 471.6
Description: The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in mouse and 90 kDa in human (glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DCs and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.

Anti-IL3RA antibody (Alexa-fluor 488)

STJ170009 100 µg
EUR 471.6
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-IL3RA antibody (Alexa-fluor 546)

STJ170010 100 µg
EUR 471.6
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-IL3RA antibody (Alexa-fluor 647)

STJ170011 100 µg
EUR 471.6
Description: IL3 exerts its biologic activity through its interaction with a cell surface receptor that consists of two subunits. The a subunit (CD123) specifically binds IL3, whereas the ß subunit is required for signaling and is common to the GMCSFR and IL5-R. 107D2.08 and 106C2.02 mAbs were obtained after mouse immunization with sorted human tonsillar PDC. Both clones strongly stain PDCs and basophils, weakly stain monocytes, CD34+ derived DCs and CD11c+ DC, while no staining is observed on T, B, NK cells as well as on mono-derived DCs. Staining with 107D2.08 and 106C2.02 mAbs are maintained on sorted PDC cultured in the presence of IL3 and CD40L, but lost when IL3 alone is added to the culture. The recognition of the IL3Ra chain by 107D2.08 and 106C2.02 was confirmed by transfection studies. 107D2.08 appeared to be the most appropriate clone for in situ studies. 107D2.08 allowed the first observation of IL3Ra+ cells in breast tumor microenvironment

Anti-CD207 antibody (Alexa-fluor 488)

STJ170014 100 µg
EUR 471.6
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-CD207 antibody (Alexa-fluor 546)

STJ170015 100 µg
EUR 471.6
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-CD207 antibody (Alexa-fluor 647)

STJ170016 100 µg
EUR 471.6
Description: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck's granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 1999, Eur.J.Immunol., 29:2695-2704; Valladeau J et al, 2000 Immunity, 12 : 71-81; Kashihara M et al, 1986, J.Invest.Derm., 87 :602-607 Ito T et al, 1999, J.Immunol., 163 :1409-1419 ;Saeland S & Valladeau J, CD207 (Langerin) Workshop reports 2002, Leukocyte-Typing VII, White Cell Diff Antigens, D. Mason et al, Eds, Oxford University Press:306-307)

Anti-IL7R antibody (Alexa-fluor 488)

STJ170020 100 µg
EUR 471.6
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-IL7R antibody (Alexa-fluor 546)

STJ170021 100 µg
EUR 471.6
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-IL7R antibody (Alexa-fluor 647)

STJ170022 100 µg
EUR 471.6
Description: The IL7-R consists of 2 chains, IL-7R known as CD127 and common cytokine receptor chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7R expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54)

Anti-Beta Tubulin/TUBB Antibody

A01857-1 100ug/vial
EUR 400.8

Anti- Beta-tubulin Monoclonal Antibody

M05613-2 100ul
EUR 476.4
Description: Mouse Monoclonal Beta-tubulin Antibody. Validated in IF, IHC, WB and tested in Human, Monkey, Mouse, Rat.

Anti- Beta-tubulin Monoclonal Antibody

M05613-3 100ul
EUR 476.4
Description: Mouse Monoclonal Beta-tubulin Antibody. Validated in IHC, WB and tested in Human, Monkey, Mouse, Rat.

anti-beta I tubulin (3F7)

LF-MA20329 100 ug
EUR 400.8
Description: Mouse monoclonal to beta I tubulin

Anti-beta I tubulin antibody

STJ96939 200 µl
EUR 236.4
Description: beta I tubulin is a protein encoded by the TUBB1 gene which is approximately 50,3 kDa. beta I tubulin is localised to the cytoplasm. It is involved in development Slit-Robo signalling, Sertoli-Sertoli cell junction dynamics, signalling in gap junctions and chaperonin-mediated protein folding. This protein falls under the beta tubulin protein family. It is one of two core protein families that heterodimerize and assemble to form microtubules. It is exists in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. beta I tubulin is expressed in the blood and cells of the nervous system. Mutations in the TUBB1 gene may result in macrothrombocytopaenia. STJ96939 was developed from clone 3F7 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody detects endogenous beta I tubulin proteins.

Anti-beta I tubulin antibody

STJ97021 200 µl
EUR 236.4
Description: Mouse monoclonal to beta I tubulin.

Anti-beta II tubulin antibody

STJ97053 200 µl
EUR 236.4
Description: beta II tubulin is a protein encoded by the TUBB2A gene which is approximately 49,9 kDa. beta II tubulin is localised to the cytoplasm and cytoskeleton. It is involved in Sertoli-Sertoli cell junction dynamics, development Slit-Robo signalling and the GPCR pathway. Tubulin is the major constituent of microtubules. Microtubules are key participants in processes such as mitosis and intracellular transport and are composed of heterodimers of alpha- and beta-tubulins. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. beta II tubulin is expressed in the nervous system, eye, skin and lung. Mutations in the TUBB2A gene may result in lung mucoepidermoid carcinoma. STJ97053 was developed from clone Mix and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous beta II tubulin protein.

Anti-beta III tubulin antibody

STJ97067 200 µl
EUR 236.4
Description: Mouse monoclonal to beta III tubulin.

Anti-beta II Tubulin antibody

STJ97122 200 µl
EUR 236.4
Description: Rabbit polyclonal to beta II Tubulin.

Anti-beta III Tubulin antibody

STJ97123 200 µl
EUR 236.4
Description: Beta III tubulin is a protein encoded by the tubb3 gene which is approximately 50,4 kDa. Beta III tubulin is localised to the cytoplasm and cytoskeleton. It is involved in signalling within gap junctions, development slit-robo signalling, sertoli-sertoli cell junction dynamics, chaperonin-mediated protein folding and tubulin folding. This protein falls under the one of two protein families, namely beta and alpha tubulin and is one of six beta tubulin isoforms. Beta III tubulin is expressed in the neurons of the peripheral and central nervous system and plays an important role in guidance and maintenance and axons. Mutations in the tubb3 gene result in congenital fibrosis of extraocular muscles type. STJ97123 was developed from clone Mix. It was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous beta III tubulin protein.

Anti-beta-tubulin antibody (HRP)

STJ97477 200 µl
EUR 236.4
Description: Beta tubulin is a protein encoded by the tubb gene which is approximately 49,7 kDa. Beta tubulin is localised to the cytoskeleton and cytoplasm. It is involved in the regulation of PLK1 activity at G2/M transition, development of Slit-Robo signalling, the innate immune system, cell cycle and organelle biogenesis and maintenance. Beta tubulin contains a highly acidic C-terminal region which can bind cations such as calcium. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain and forms part of the cytoskeleton. Beta tubulin is ubiquitously expressed in the spleen, thymus and immature brain. Mutations in the tubb gene result in complex cortical dysplasia with other brain malformations. Mutations can also cause congenital symmetric circumferential, an autosomal dominant disease which results in multiple rings of folded skin mostly affecting the limbs. STJ97477 was developed from clone 5G3. The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous beta tubulin (HRP Conjugated).

Anti-Tubulin, beta III antibody

STJ120262 100 µl
EUR 631.2

Anti-Beta III Tubulin antibody

STJ16100477 100 µg
EUR 487.2

Anti-beta II Tubulin (1F9)

YF-MA17277 100 ug
EUR 435.6
Description: Mouse monoclonal to beta II Tubulin

Anti-beta II Tubulin (1G3)

YF-MA17278 100 ug
EUR 435.6
Description: Mouse monoclonal to beta II Tubulin

alpha Tubulin (alpha Tubulin) Antibody (PE/ATTO 594)

abx446854-100ug 100 ug
EUR 710.4

XFD594 tyramide reagent *Same Structure to Alexa Fluor™ 594 tyramide*

11082 200 slides
EUR 222

Rabbit Anti-Rat IgG (H+L)-Alexa 594 Fluor conjugate (adsorbed with human IgG)

50337 0.5 ml
EUR 270

XFD594 Anti-human CD47 Antibody *HI172, XFD594 Same Structure to Alexa Fluor™ 594*

10471170 100 tests
EUR 245

XFD594 Anti-human CD47 Antibody *HI172, XFD594 Same Structure to Alexa Fluor™ 594*

10471171 500 tests
EUR 918

XFD594 Anti-human CD52 Antibody *HI186, XFD594 Same Structure to Alexa Fluor™ 594*

10520170 100 tests
EUR 245

XFD594 Anti-human CD52 Antibody *HI186, XFD594 Same Structure to Alexa Fluor™ 594*

10520171 500 tests
EUR 918

XFD594 Anti-human CD53 Antibody *HI29, XFD594 Same Structure to Alexa Fluor™ 594*

10530170 100 tests
EUR 245

XFD594 Anti-human CD53 Antibody *HI29, XFD594 Same Structure to Alexa Fluor™ 594*

10530171 500 tests
EUR 918

XFD594 Anti-human CD53 Antibody *HI36, XFD594 Same Structure to Alexa Fluor™ 594*

10531170 100 tests
EUR 245

XFD594 Anti-human CD53 Antibody *HI36, XFD594 Same Structure to Alexa Fluor™ 594*

10531171 500 tests
EUR 918

XFD594 Anti-human CD55 Antibody *HI55a, XFD594 Same Structure to Alexa Fluor™ 594*

10550170 100 tests
EUR 245

XFD594 Anti-human CD55 Antibody *HI55a, XFD594 Same Structure to Alexa Fluor™ 594*

10550171 500 tests
EUR 918

XFD594 Anti-human CD56 Antibody *My31, XFD594 Same Structure to Alexa Fluor™ 594*

10562160 100 tests
EUR 245

XFD594 Anti-human CD56 Antibody *My31, XFD594 Same Structure to Alexa Fluor™ 594*

10562161 500 tests
EUR 918

XFD594 Anti-human CD57 Antibody *HI57a, XFD594 Same Structure to Alexa Fluor™ 594*

10570170 100 tests
EUR 245

XFD594 Anti-human CD57 Antibody *HI57a, XFD594 Same Structure to Alexa Fluor™ 594*

10570171 500 tests
EUR 918

XFD594 Anti-human CD58 Antibody *HI58a, XFD594 Same Structure to Alexa Fluor™ 594*

10580170 100 tests
EUR 245

XFD594 Anti-human CD58 Antibody *HI58a, XFD594 Same Structure to Alexa Fluor™ 594*

10580171 500 tests
EUR 918

XFD594 Anti-human CD62 Antibody *HI62E, XFD594 Same Structure to Alexa Fluor™ 594*

10620170 100 tests
EUR 245

XFD594 Anti-human CD62 Antibody *HI62E, XFD594 Same Structure to Alexa Fluor™ 594*

10620171 500 tests
EUR 918

XFD594 Anti-human CD62l Antibody *HI62L, XFD594 Same Structure to Alexa Fluor™ 594*

10621170 100 tests
EUR 245

XFD594 Anti-human CD62l Antibody *HI62L, XFD594 Same Structure to Alexa Fluor™ 594*

10621171 500 tests
EUR 918

XFD594 Anti-human CD62p Antibody *HI62P, XFD594 Same Structure to Alexa Fluor™ 594*

10622170 100 tests
EUR 245

XFD594 Anti-human CD62p Antibody *HI62P, XFD594 Same Structure to Alexa Fluor™ 594*

10622171 500 tests
EUR 918

XFD594 Anti-human CD64 Antibody *10.1, XFD594 Same Structure to Alexa Fluor™ 594*

10640170 100 tests
EUR 245

XFD594 Anti-human CD64 Antibody *10.1, XFD594 Same Structure to Alexa Fluor™ 594*

10640171 500 tests
EUR 918

XFD594 Anti-human CD69 Antibody *FN50, XFD594 Same Structure to Alexa Fluor™ 594*

10690170 100 tests
EUR 547

XFD594 Anti-human CD69 Antibody *FN50, XFD594 Same Structure to Alexa Fluor™ 594*

10690171 500 tests
EUR 2051

XFD594 Anti-human CD71 Antibody *HI160, XFD594 Same Structure to Alexa Fluor™ 594*

10710170 100 tests
EUR 245

XFD594 Anti-human CD71 Antibody *HI160, XFD594 Same Structure to Alexa Fluor™ 594*

10710171 500 tests
EUR 918

XFD594 Anti-human CD71 Antibody *HI166, XFD594 Same Structure to Alexa Fluor™ 594*

10711170 100 tests
EUR 245

XFD594 Anti-human CD71 Antibody *HI166, XFD594 Same Structure to Alexa Fluor™ 594*

10711171 500 tests
EUR 918

XFD594 Anti-human CD72 Antibody *3F3, XFD594 Same Structure to Alexa Fluor™ 594*

10720170 100 tests
EUR 547

XFD594 Anti-human CD72 Antibody *3F3, XFD594 Same Structure to Alexa Fluor™ 594*

10720171 500 tests
EUR 2051

XFD594 Anti-human CD73 Antibody *AD2, XFD594 Same Structure to Alexa Fluor™ 594*

10730170 100 tests
EUR 547

XFD594 Anti-human CD73 Antibody *AD2, XFD594 Same Structure to Alexa Fluor™ 594*

10730171 500 tests
EUR 2051

XFD594 Anti-human CD5 Antibody *HISM2, XFD594 Same Structure to Alexa Fluor™ 594*

10050170 100 tests
EUR 245

XFD594 Anti-human CD5 Antibody *HISM2, XFD594 Same Structure to Alexa Fluor™ 594*

10050171 500 tests
EUR 918

XFD594 Anti-human CD5 Antibody *L17F12, XFD594 Same Structure to Alexa Fluor™ 594*

10051170 100 tests
EUR 245

XFD594 Anti-human CD5 Antibody *L17F12, XFD594 Same Structure to Alexa Fluor™ 594*

10051171 500 tests
EUR 918

XFD594 Anti-human CD5 Antibody *UCHT2, XFD594 Same Structure to Alexa Fluor™ 594*

10052170 100 tests
EUR 245

XFD594 Anti-human CD5 Antibody *UCHT2, XFD594 Same Structure to Alexa Fluor™ 594*

10052171 500 tests
EUR 918

XFD594 Anti-human CD6 Antibody *HI210, XFD594 Same Structure to Alexa Fluor™ 594*

10060170 100 tests
EUR 245

XFD594 Anti-human CD6 Antibody *HI210, XFD594 Same Structure to Alexa Fluor™ 594*

10060171 500 tests
EUR 918

XFD594 Anti-human CD7 Antibody *HIT7, XFD594 Same Structure to Alexa Fluor™ 594*

10070170 100 tests
EUR 245

XFD594 Anti-human CD7 Antibody *HIT7, XFD594 Same Structure to Alexa Fluor™ 594*

10070171 500 tests
EUR 918

XFD594 Anti-human CD8 Antibody *HIT8a, XFD594 Same Structure to Alexa Fluor™ 594*

10080170 100 tests
EUR 245

XFD594 Anti-human CD8 Antibody *HIT8a, XFD594 Same Structure to Alexa Fluor™ 594*

10080171 500 tests
EUR 918

XFD594 Anti-human CD8 Antibody *SK1, XFD594 Same Structure to Alexa Fluor™ 594*

10081170 100 tests
EUR 245

XFD594 Anti-human CD8 Antibody *SK1, XFD594 Same Structure to Alexa Fluor™ 594*

10081171 500 tests
EUR 918

XFD594 Anti-human CD9 Antibody *HI9a, XFD594 Same Structure to Alexa Fluor™ 594*

10090170 100 tests
EUR 245

XFD594 Anti-human CD9 Antibody *HI9a, XFD594 Same Structure to Alexa Fluor™ 594*

10090171 500 tests
EUR 918

XFD594 Anti-human CD10 Antibody *HI10a, XFD594 Same Structure to Alexa Fluor™ 594*

10100170 100 tests
EUR 245

XFD594 Anti-human CD10 Antibody *HI10a, XFD594 Same Structure to Alexa Fluor™ 594*

10100171 500 tests
EUR 918

XFD594 Anti-human CD11a Antibody *HI111, XFD594 Same Structure to Alexa Fluor™ 594*

10110170 100 tests
EUR 245

XFD594 Anti-human CD11a Antibody *HI111, XFD594 Same Structure to Alexa Fluor™ 594*

10110171 500 tests
EUR 918

XFD594 Anti-human CD11b Antibody *HI11b, XFD594 Same Structure to Alexa Fluor™ 594*

10111170 100 tests
EUR 245

XFD594 Anti-human CD11b Antibody *HI11b, XFD594 Same Structure to Alexa Fluor™ 594*

10111171 500 tests
EUR 918

XFD594 Anti-human CD11b Antibody *ICRF44, XFD594 Same Structure to Alexa Fluor™ 594*

10112170 100 tests
EUR 245

XFD594 Anti-human CD11b Antibody *ICRF44, XFD594 Same Structure to Alexa Fluor™ 594*

10112171 500 tests
EUR 918

XFD594 Anti-human CD11c Antibody *3.9, XFD594 Same Structure to Alexa Fluor™ 594*

10113170 100 tests
EUR 245

XFD594 Anti-human CD11c Antibody *3.9, XFD594 Same Structure to Alexa Fluor™ 594*

10113171 500 tests
EUR 918

XFD594 Anti-human CD13 Antibody *WM15, XFD594 Same Structure to Alexa Fluor™ 594*

10130170 100 tests
EUR 245

XFD594 Anti-human CD13 Antibody *WM15, XFD594 Same Structure to Alexa Fluor™ 594*

10130171 500 tests
EUR 918

XFD594 Anti-human CD14 Antibody *61D3, XFD594 Same Structure to Alexa Fluor™ 594*

10141170 100 tests
EUR 245

XFD594 Anti-human CD14 Antibody *61D3, XFD594 Same Structure to Alexa Fluor™ 594*

10141171 500 tests
EUR 918

XFD594 Anti-human CD15 Antibody *HI98, XFD594 Same Structure to Alexa Fluor™ 594*

10150170 100 tests
EUR 296

XFD594 Anti-human CD15 Antibody *HI98, XFD594 Same Structure to Alexa Fluor™ 594*

10150171 500 tests
EUR 1020

XFD594 Anti-human CD16 Antibody *HI16a, XFD594 Same Structure to Alexa Fluor™ 594*

10160170 100 tests
EUR 245

XFD594 Anti-human CD16 Antibody *HI16a, XFD594 Same Structure to Alexa Fluor™ 594*

10160171 500 tests
EUR 918

XFD594 Anti-human CD16 Antibody *3G8, XFD594 Same Structure to Alexa Fluor™ 594*

10161170 100 tests
EUR 245

XFD594 Anti-human CD16 Antibody *3G8, XFD594 Same Structure to Alexa Fluor™ 594*

10161171 500 tests
EUR 918

XFD594 Anti-human CD18 Antibody *HI18a, XFD594 Same Structure to Alexa Fluor™ 594*

10180170 100 tests
EUR 245

XFD594 Anti-human CD18 Antibody *HI18a, XFD594 Same Structure to Alexa Fluor™ 594*

10180171 500 tests
EUR 918

XFD594 Anti-human CD19 Antibody *HI19a, XFD594 Same Structure to Alexa Fluor™ 594*

10190170 100 tests
EUR 245

XFD594 Anti-human CD19 Antibody *HI19a, XFD594 Same Structure to Alexa Fluor™ 594*

10190171 500 tests
EUR 918

XFD594 Anti-human CD19 Antibody *SJ25C1, XFD594 Same Structure to Alexa Fluor™ 594*

10191170 100 tests
EUR 245

XFD594 Anti-human CD19 Antibody *SJ25C1, XFD594 Same Structure to Alexa Fluor™ 594*

10191171 500 tests
EUR 918

XFD594 Anti-human CD19 Antibody *HIB19, XFD594 Same Structure to Alexa Fluor™ 594*

10192170 100 tests
EUR 245

XFD594 Anti-human CD19 Antibody *HIB19, XFD594 Same Structure to Alexa Fluor™ 594*

10192171 500 tests
EUR 918

XFD594 Anti-human CD19 Antibody *4G7, XFD594 Same Structure to Alexa Fluor™ 594*

10193170 100 tests
EUR 245

XFD594 Anti-human CD19 Antibody *4G7, XFD594 Same Structure to Alexa Fluor™ 594*

10193171 500 tests
EUR 918

XFD594 Anti-mouse CD19 Antibody *1D3, XFD594 Same Structure to Alexa Fluor™ 594*

10194160 100 tests
EUR 245

XFD594 Anti-mouse CD19 Antibody *1D3, XFD594 Same Structure to Alexa Fluor™ 594*

10194161 500 tests
EUR 918

XFD594 Anti-human CD20 Antibody *HI20a, XFD594 Same Structure to Alexa Fluor™ 594*

10201170 100 tests
EUR 245

XFD594 Anti-human CD20 Antibody *HI20a, XFD594 Same Structure to Alexa Fluor™ 594*

10201171 500 tests
EUR 918

XFD594 Anti-human CD21 Antibody *HI21a, XFD594 Same Structure to Alexa Fluor™ 594*

10210170 100 tests
EUR 245

XFD594 Anti-human CD21 Antibody *HI21a, XFD594 Same Structure to Alexa Fluor™ 594*

10210171 500 tests
EUR 918

XFD594 Anti-human CD22 Antibody *HIB22, XFD594 Same Structure to Alexa Fluor™ 594*

10220170 100 tests
EUR 245

XFD594 Anti-human CD22 Antibody *HIB22, XFD594 Same Structure to Alexa Fluor™ 594*

10220171 500 tests
EUR 918

XFD594 Anti-human CD101 Antibody *BB27, XFD594 Same Structure to Alexa Fluor™ 594*

11010160 100 tests
EUR 547

XFD594 Anti-human CD101 Antibody *BB27, XFD594 Same Structure to Alexa Fluor™ 594*

11010161 500 tests
EUR 2051

XFD594 Anti-human CD107 Antibody *H4B4, XFD594 Same Structure to Alexa Fluor™ 594*

11071160 100 tests
EUR 547

XFD594 Anti-human CD107 Antibody *H4B4, XFD594 Same Structure to Alexa Fluor™ 594*

11071161 500 tests
EUR 2051

XFD594 Anti-human CD109 Antibody *W7C5, XFD594 Same Structure to Alexa Fluor™ 594*

11090160 100 tests
EUR 547

XFD594 Anti-human CD109 Antibody *W7C5, XFD594 Same Structure to Alexa Fluor™ 594*

11090161 500 tests
EUR 2051

XFD594 Anti-human CD111 Antibody *R1.302, XFD594 Same Structure to Alexa Fluor™ 594*

11110160 100 tests
EUR 547

XFD594 Anti-human CD111 Antibody *R1.302, XFD594 Same Structure to Alexa Fluor™ 594*

11110161 500 tests
EUR 2051

XFD594 Anti-human CD112 Antibody *R2.525, XFD594 Same Structure to Alexa Fluor™ 594*

11120160 100 tests
EUR 547

XFD594 Anti-human CD112 Antibody *R2.525, XFD594 Same Structure to Alexa Fluor™ 594*

11120161 500 tests
EUR 2051

XFD594 Anti-human CD114 Antibody *LMM741, XFD594 Same Structure to Alexa Fluor™ 594*

11140160 100 tests
EUR 547

XFD594 Anti-human CD114 Antibody *LMM741, XFD594 Same Structure to Alexa Fluor™ 594*

11140161 500 tests
EUR 2051

XFD594 Anti-human CD116 Antibody *4H1, XFD594 Same Structure to Alexa Fluor™ 594*

11160160 100 tests
EUR 547

XFD594 Anti-human CD116 Antibody *4H1, XFD594 Same Structure to Alexa Fluor™ 594*

11160161 500 tests
EUR 2051

XFD594 Anti-human CD117 Antibody *104D2, XFD594 Same Structure to Alexa Fluor™ 594*

11170170 100 tests
EUR 547

XFD594 Anti-human CD117 Antibody *104D2, XFD594 Same Structure to Alexa Fluor™ 594*

11170171 500 tests
EUR 2051

XFD594 Anti-human CD120a Antibody *H398, XFD594 Same Structure to Alexa Fluor™ 594*

11200160 100 tests
EUR 547

XFD594 Anti-human CD120a Antibody *H398, XFD594 Same Structure to Alexa Fluor™ 594*

11200161 500 tests
EUR 2051

XFD594 Anti-human CD122 Antibody *TU27, XFD594 Same Structure to Alexa Fluor™ 594*

11220160 100 tests
EUR 547

XFD594 Anti-human CD122 Antibody *TU27, XFD594 Same Structure to Alexa Fluor™ 594*

11220161 500 tests
EUR 2051

Nowatorski automatyczny check immunologiczny dla przeciwciał NY-ESO-1 i XAGE1 w surowicy w skojarzonym przewidywaniu odpowiedzi na terapię przeciw zaprogramowanej komórkowej – 1 w niedrobnokomórkowym raku płuca

Tło:  anty-zaprogramowana śmierć komórki 1 (PD-1)  przeciwciała  (ABS) są podstawowymi lekami w niedrobnokomórkowym rakiem płuc obróbki (NSCLC); jednakże korzyści kliniczne związane z monoterapią anty-PD-1 są ograniczone. Poinformowaliśmy, że przeciwciała Abs w surowicy przeciwko antygenom jąder nowotworowych NY-ESO-1 i XAGE1 przewidywały korzyści kliniczne. Naszym celem było opracowanie w pełni zautomatyzowanego systemu immunologicznego mierzącego NY-ESO-1 / XAGE1 Abs.

Metody:  Surowice od 30 pacjentów z NSCLC przed monoterapią anty-PD-1 poddano reakcji z kulkami magnetycznymi pokrytymi rekombinowanym białkiem NY-ESO-1 lub syntetycznym peptydem XAGE1. Dodano Ab skoniugowane z ALP i substrat chemiluminescencyjny i zmierzono luminescencję. Procedury te zautomatyzowano przy użyciu immunoenzymatycznego testu chemiluminescencyjnego o wysokiej czułości (HISCL TM ). Stabilność NY-ESO-1 / XAGE1 Ab badano w różnych warunkach. Dokładność przewidywania odpowiedzi oceniano przy użyciu obszaru pod krzywą operacyjną odbiornika (AUROC).

Wyniki:  HISCL wykrył specyficzne przeciwciała NY-ESO-1 / XAGE1 w surowicy, których poziomy w testach ELISA i HISCL były silnie skorelowane. Poziomy Ab w HISCL były stabilne w czterech temperaturach, pięciu cyklach zamrażania / rozmrażania i długotrwałym przechowywaniu; poziomy nie były zakłócane przez zwykłe składniki krwi. Poziomy Ab u 15 osób z odpowiedzią na NSCLC na monoterapię anty-PD-1 były istotnie wyższe niż u niereagujących i zdrowych dawców. AUROC był najwyższy (0,91; 95% CI, 0,78-1,0) w predykcji kombinacyjnej z NY-ESO-1 / XAGE1 Abs.

Wniosek:  Nasz system testów immunologicznych jest przydatny do przewidywania korzyści klinicznych związanych z terapią immunopunktową NSCLC

Korelacja odpowiedzi na szczepionki

Wprowadzenie:  Reakcja humoralna na szczepienia jest bardzo zróżnicowana u poszczególnych osób. Nie ma dostępnych danych dotyczących korelacji między odpowiedziami na różne szczepionki. W tym badaniu zbadaliśmy korelację   odpowiedzi przeciwciał między rutynowymi antygenami szczepionkowymi u niemowląt.

Metody:  Jeden i siedem miesięcy po szczepieniach 6-miesięcznych i miesiąc po szczepieniach 12-miesięcznych   stężenia przeciwciał przeciwko błonicy, tężcowi, krztuścowi, polio (serotypy 1-3),  Haemophilus influenzae  typ b (Hib), pneumokokom (13 serotypy), meningokoki C, odrę, świnkę i różyczkę za pomocą multipleksowych testów immunologicznych opartych na kulkach fluorescencyjnych. Dla korelacji   odpowiedzi przeciwciał obliczono współczynniki korelacji rang Spearmana (ρ) z 95% przedziałami ufności (CI) między odpowiedziami na każdy antygen szczepionkowy.

Wyniki:  Korelacja pomiędzy stężeniami przeciwciał przeciwko szczepieniom kończącym się w wieku 6 miesięcy była wyższa po miesiącu w porównaniu z siedmioma miesiącami po szczepieniu. Najsilniejsze korelacje w obu punktach czasowych zaobserwowano między   odpowiedziami przeciwciał na różne serotypy polio, określone serotypy pneumokokowe oraz między odpowiedziami na antygeny szczepionki przeciw błonicy i pneumokokom (sprzężonym z toksoidem błoniczym). Korelacja między odpowiedziami na tężec, Hib, krztusiec, polio i inne antygeny szczepionkowe była słaba. Korelacja między  przeciwciałami odpowiedzi na 12-miesięczne antygeny szczepionkowe były słabsze niż na 6-miesięczne antygeny zawarte w szczepionce i istniała ujemna korelacja między odpowiedziami na szczepionkę przeciw odrze, śwince, różyczce i nieżywymi antygenami szczepionkowymi (meningokok C, tężec i Hib). Występowała tylko słaba korelacja między   odpowiedziami przeciwciał na szczepionki tego samego typu (np. Szczepionki ze sprzężonym polisacharydem lub toksoidem).

Wniosek:  Korelacja między   odpowiedziami przeciwciał na podobne antygeny w tej samej szczepionce (np. Różne serotypy bakterii lub wirusa), jak również odpowiedzi na antygeny sprzężone z podobnymi białkami nośnikowymi są silne. W przeciwieństwie do tego, korelacja między odpowiedziami na inne szczepionki jest słaba. Pomiar   odpowiedzi przeciwciał na jeden lub kilka antygenów szczepionkowych nie zapewnia zatem wiarygodnego zastępczego markera odpowiedzi na niepowiązane szczepionki.

Białko mitochondrialne CMPK2 reguluje tworzenie komórek piankowatych wzmocnionych przez IFN alfa, potencjalnie przyczyniając się do przedwczesnej miażdżycy tętnic w SLE

Wstęp:  Przedwczesna miażdżyca tętnic występuje u chorych na SLE; jednak mechanizmy pozostają niejasne. Zarówno maszyneria mitochondrialna, jak i prozapalna cytokina interferon alfa (IFN-α) potencjalnie przyczyniają się do procesów aterogennych w SLE. W tym miejscu badamy rolę mitochondrialnej białkowej kinazy cytydyny / monofosforanu urydyny 2 (CMPK2) w zdarzeniach pro-miażdżycowych, w których pośredniczy IFN-α.

Metody:  Pomiary komórek piankowych przeprowadzono za pomocą barwienia czerwieni oleistej O, wychwytu Dil-oxLDL i metody BODIPY. Te poziomy mRNA i białka były mierzone qPCR i Western blot, odpowiednio. Izolację komórek T CD4 + i monocytów przeprowadzono za pomocą przeciwciał  monoklonalnych  skoniugowanych  z mikrokulkami. Manipulację ekspresją białka prowadzono albo przez knock-down małej interferencji RNA (siRNA), albo knock-out CRISPR / Cas9. Ekspresję mitochondrialnych reaktywnych kind tlenu (mtROS) określono za pomocą cytometrii przepływowej i mikroskopii konfokalnej.

Wyniki:  Tworzenie komórek piankowatych indukowane przez IFN-α i pobór Dil-oxLDL przez makrofagi. Oprócz IFN-α, kilka czynników wyzwalających miażdżycę tętnic, w tym trombina i IFN-γ, może indukować ekspresję CMPK2, która była podwyższona w limfocytach T CD4 + i monocytach CD14 + izolowanych od pacjentów z SLE w porównaniu z tymi wyizolowanymi z kontroli. Analiza podfrakcji komórkowych ujawniła, że ​​CMPK2 był obecny zarówno we frakcji mitochondrialnej, jak i cytozolowej. Ekspresja CMPK2 indukowana przez IFN-α była hamowana przez inhibitory kinazy janusowej (JAK) half i kinazy tyrozynowej 2 (Tyk2). Zarówno knockdown, jak i knockout CMPK2 osłabiły tworzenie komórek piankowatych, w którym pośredniczy IFN-α, co obejmowało zmniejszenie ekspresji receptora zmiatacza klasy A (SR-A). CMPK2 reguluje również mtROS ze wzmocnionym IFN-α produkcja i aktywacja inflamasomu.

Wnioski:  Badanie sugeruje, że CMPK2 odgrywa ważną rolę w pro-miażdżycowym działaniu IFN-α.

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